pDUAL OCLN (GFP)

LOCUS       pDUAL OCLN (GFP)              11777 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral expression construct encoding Occludin and GFP from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    pDUAL OCLN (GFP)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11777)
  TITLE     Grove lab plasmids
REFERENCE   2  (bases 1 to 11777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             16..649
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    696..821
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1318..1551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1736..1780
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2065..2182
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2335..2742
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     RBS             2799..2807
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(4543..4563)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4961..4972)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5008..5518
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5437..5455
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      5533..5542
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5539..6258
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             5539..6258
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5584..5605)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5845..5864)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6192..6213
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6354..6587
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6612..6630)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6613..6630)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6920..6939)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7153..7172)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7272..7294
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7332..7350)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7418..7522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7523..8383
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7741..7760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8554..9142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9043..9062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9296..9313
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9383..9403)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9388..9717
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9568..9703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9630..9649
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          10851..10916
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11046..11066
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11491..11625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11528..11547)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11582..11601
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
       61 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
      121 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
      181 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
      241 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      301 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
      361 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      421 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      481 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      541 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      601 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      661 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      721 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      781 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      841 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      901 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      961 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1021 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1081 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1141 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1201 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1261 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1321 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1381 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1441 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1501 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1561 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1621 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1681 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1741 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1801 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1861 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1921 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1981 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2041 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2101 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2161 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat
     2221 cgataagctt gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc
     2281 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     2341 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     2401 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     2461 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     2521 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     2581 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     2641 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     2701 tcactcggcg cgccagtcct ccgacagact gagtcgcccg gggggatcca ctagtgcggc
     2761 cgcgccgcca ccatgtcatc caggcctctt gaaagtccac ctccttacag gcctgatgaa
     2821 ttcaaaccga atcattatgc accaagcaat gacatatatg gtggagagat gcatgttcga
     2881 ccaatgctct ctcagccagc ctactctttt tacccagaag atgaaattct tcacttctac
     2941 aaatggacct ctcctccagg agtgattcgg atcctgtcta tgctcattat tgtgatgtgc
     3001 attgccatct ttgcctgtgt ggcctccacg cttgcctggg acagaggcta tggaacttcc
     3061 cttttaggag gtagtgtagg ctacccttat ggaggaagtg gctttggtag ctacggaagt
     3121 ggctatggct atggctatgg ttatggctat ggctacggag gctatacaga cccaagagca
     3181 gcaaagggct tcatgttggc catggctgcc ttttgtttca ttgccgcgtt ggtgatcttt
     3241 gttaccagtg ttataagatc tgaaatgtcc agaacaagaa gatactactt aagtgtgata
     3301 atagtgagtg ctatcctggg catcatggtg tttattgcca caattgtcta tataatggga
     3361 gtgaacccaa ctgctcagtc ttctggatct ctatatggtt cacaaatata tgccctctgc
     3421 aaccaatttt atacacctgc agctactgga ctctacgtgg atcagtattt gtatcactac
     3481 tgtgttgtgg atccccagga ggccattgcc attgtactgg ggttcatgat tattgtggct
     3541 tttgctttaa taattttctt tgctgtgaaa actcgaagaa agatggacag gtatgacaag
     3601 tccaatattt tgtgggacaa ggaacacatt tatgatgagc agccccccaa tgtcgaggag
     3661 tgggttaaaa atgtgtctgc aggcacacag gacgtgcctt cacccccatc tgactatgtg
     3721 gaaagagttg acagtcccat ggcatactct tccaatggca aagtgaatga caagcggttt
     3781 tatccagagt cttcctataa atccacgccg gttcctgaag tggttcagga gcttccatta
     3841 acttcgcctg tggatgactt caggcagcct cgttacagca gcggtggtaa ctttgagaca
     3901 ccttcaaaaa gagcacctgc aaagggaaga gcaggaaggt caaagagaac agagcaagat
     3961 cactatgaga cagactacac aactggcggc gagtcctgtg atgagctgga ggaggactgg
     4021 atcagggaat atccacctat cacttcagat caacaaagac aactgtacaa gaggaatttt
     4081 gacactggcc tacaggaata caagagctta caatcagaac ttgatgagat caataaagaa
     4141 ctctcccgtt tggataaaga attggatgac tatagagaag aaagtgaaga gtacatggct
     4201 gctgctgatg aatacaatag actgaagcaa gtgaagggat ctgcagatta caaaagtaag
     4261 aagaatcatt gcaagcagtt aaagagcaaa ttgtcacaca tcaagaagat ggttggagac
     4321 tatgatagac agaaaacata gaaggctgat gccaagttgt ttgagaaatt aagtatctga
     4381 catctctgca atcttctcag aaggcaaatg actttggacc ataaccccgg aagccaaacc
     4441 tctgtgagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
     4501 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4561 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4621 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4681 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4741 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4801 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4861 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     4921 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4981 ccttccttcc cgcggtcgaa ttcccacggg gttggggttg cgccttttcc aaggcagccc
     5041 tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga
     5101 ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac
     5161 ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg
     5221 gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga
     5281 cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagtgg
     5341 ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg
     5401 tggggcggta gtgtgggccc tgttcctgcc cgcacggtgt tccgcattct gcaagcctcc
     5461 ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccagga
     5521 cgcgtaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     5581 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     5641 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     5701 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     5761 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     5821 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     5881 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     5941 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt ctatatcacc
     6001 gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa catcgaggac
     6061 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     6121 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     6181 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     6241 gacgagctgt acaagtaaac tcgagggaat taattcgagc tcggtacctt taagaccaat
     6301 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6361 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     6421 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6481 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6541 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta
     6601 attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
     6661 taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta
     6721 ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga
     6781 accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc
     6841 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     6901 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     6961 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     7021 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     7081 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg
     7141 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     7201 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     7261 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     7321 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     7381 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7441 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7501 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7561 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7621 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7681 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7741 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7801 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7861 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7921 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7981 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8041 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8101 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8161 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8221 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8281 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8341 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8401 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8461 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8521 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8581 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8641 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8701 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8761 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8821 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8881 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8941 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9001 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9061 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9121 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9181 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9241 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9301 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     9361 gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     9421 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     9481 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9541 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9601 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9661 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9721 cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     9781 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     9841 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     9901 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     9961 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
    10021 aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
    10081 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
    10141 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
    10201 actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
    10261 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt
    10321 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
    10381 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
    10441 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
    10501 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
    10561 accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa
    10621 ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg
    10681 tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta
    10741 ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct
    10801 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
    10861 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
    10921 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
    10981 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
    11041 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
    11101 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
    11161 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta
    11221 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata
    11281 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta
    11341 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
    11401 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
    11461 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
    11521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    11581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    11641 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    11701 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    11761 atttgttaaa tatgtac
//